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Animal and vegetable fats and oils
  • ISO 660-2020 pdf download.Animal and vegetable fats and oils 一 Determination of acid value and acidity. This document specifies three methods (two titrimetric and one potentiometric) for the determination of acidity in animal and vegetable fats and oils, hereinafter referred to as “fats”. The acidity is expressed preferably as acid value or, alternatively, as acidity calculated conventionally. This document is applicable to refined and crude vegetable or animal fats and oils, soap stock fatty acids or technical fatty acids. It does not apply to waxes. Since the methods are completely non-specific, they do not apply to differentiating between mineral acids, free fatty acids and other organic acids. The acid value, therefore, includes any mineral acids that are present. Milk and milk products (or fat coming from milk and milk products) are excluded from the Scope of this document. 2 Normative references The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 385, Laboratory glass ware — Burettes ISO 661, Animal and vegetable…

  • ISO 12966-1-2014 pdf download.Animal and vegetable fats and oils – Gas chromatography of fatty acid methyl esters一 Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters. This part of ISO 12966 gives an overview of the gas chromatographic determination of fatty acids, free and bound, in animal and vegetable fats and oils following their conversion to fatty acid methyl esters (FAMEs). The qualitative and quantitative determination of the composition of fatty acids by gas liquid chromatography (GLC) is a widely used application in lipid analysis. It is used for the characterization of fats and oils, or fatty foodstuffs after the extraction of the oil from the matrix. The bound fatty acids of the triacylglycerols (TAGs) and, depending on the esterification method, the free fatty acids (FFA) and other lipids, are converted to fatty acid methyl esters (FAMES), which are determined by capillary gas chromatography. Depending on the number of different fatty acids (theoretically more than 50 different fatty acids can be present) capillary columns with a length of 10 m to 100 m are used for a separation. The GLC of FAMEs is applicable to all natural and synthetic mixtures of tri-, di- and monoacylglycerols,to fatty acid…