BS ISO 18074:2015 pdf free.Textiles – ldentification of some animal fibres by DNA analysis method一Cashmere, wool, yak and their blends.
BS ISO 18074 specifies a testing method for DNA analysis of some animal fibres to identify cashmere, wool, yak, and their blends by using extraction, amplification by the polymerase chain reaction (PCR) method and DNA detection processes.
This International Standard is applicable to cashmere, yak, and wool and their blends as a qualitative method.
2 Caution
Test results for fibre identification by the DNA analysis method can be obtained with a high accuracy for the above-mentioned textile products which were processed at lower dye concentration levels or dyed in light colours.
However, when such textile products were processed under severe conditions or high temperatures, the mitochondrial DNA could have been damaged. In such cases, identification can be difficult because amplification of DNA by PCR cannot take place. If textile products were contaminated by using products from another species, such as cashmere grease on wool fibres, this situation may be solved by checking using microscopy techniques.
3 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods ISO 8655-2, Piston-operated volumetric apparatus — Part 2: Piston pipettes
4 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
4.1 DNA deoxyribonucleic acid, that exists in nuclei and in mitochondria of animal fibre cells and is composed of a linear array of 4 bases (Adenine: A, Thymine: T, Guanine: G and Cytosine: C)
Note ito entry: The DNA sequence is identical and intrinsic for each animal fibre.
4.2 animal fibres cashmere, wool, or yak fibres.BS ISO 18074 pdf free download.
Tags: ldentification of some animal fibres by DNA analysis method
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