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AS 4276.1:2021 pdf download
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AS 4276.1:2021 pdf download.Water microbiology Method 1: Water quality一General requirements and guidance for microbiological examinations by culture (ISO 8199:2018, MOD).
This document specifies requirements and gives guidance for performing the manipulations common to each culture technique for the microbiological examination of water, particularly the preparation of samples, culture n’,edia, and general apparatus and glassware, unless otherwise required in the specific standard. It also describes the various techniques available for detection and enumeration by culture and the criteria for determining which technique is appropriate.
This document is mainly intended for examinations for bacteria, yeasts and moulds, but some aspects are also applicable to bacteriophages, viruses and parasites. It excludes techniques not based on culturing microorganisms, such as polymerase chain reaction (PCRJ methods.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 7704, Water quality — Evaluation of membrane filters used for microbiological analyses
ISO 11133, Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 19458, Water quality — Sampling for microbiological analysis
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www org/b.p
— IEC Electroped ia: available at http://www.electropedia.org/
3.1 accuracy
closeness of agreement between a test result and the accepted reference value
[SOURCE: ISO 6107-8:1993, 1, modified — The note has been deleted.]
3.2 bias
difference between the expectation of the test results and an accepted reference value
[SOURCE: ISO 5725-1:1994, 3.8. modified — Note 1 to entry has been deleted.]
3.3 confirmed count
count (3.4) corrected for not confirmed presumptive counts (3.9) by further testing of the presumptive objects
3.4 count
<microbiology> observed number of objects such as colonies or cells determined by direct counting, or most probable number (MPN) estimation based on statistical calculation using the number of positive units or presumptive positive units in a dilution series of a test sample (3.16)
[SOURCE: ISO 6107-6:2004, 22, modified — ‘or presumptive positive units” has been added.J
3.5 detection level
minimum concentration of organisms that produce evidence of growth with a probability of p = 0,95 when inoculated into a specified culture medium and incubated under defined conditions
Note I to entry: The theoretical level that conforms to this definition is an average of three viable cells in an inoculum volume.
[SOURCE: ISO 13843:2017, 3.101
3.6 intralaboratory reproducibility intermediate precision
closeness of agreement between test results obtained with the same method on the same or similar test materials in the same laboratory with different operators using different equipment.AS 4276.1 pdf download.


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