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BS ISO 21285:2019 pdf free
HomeISO StandardsBS ISO 21285:2019 pdf free
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BS ISO 21285:2019 pdf free.Soil quality – Inhibition of reproduction of the soil mite (Hypoaspis aculeifer) by soil contaminants.
Approximately 0,3 % to 1,0 % calcium carbonate (CaCO3, pulverised, analytical grade) are necessary to get a pH of 6,0 ± 0,5.
NOTE 1 Taking the properties of highly non-polar (log I(> 2) or ionizing substances into account, 5 % of peat have proven to be sufficient for maintaining the desired structure of the artificial soil.
NOTE 2 It has been demonstrated that Hypoaspis aculeifer can comply with the validity criteria even on reproduction when tested In fIeld soils with lower organic carbon content (e.g. 2,7 %), and there is experience that this can be achieved In artificial soil with 5 % peat. Therefore, it is not necessary before using such a soil in a definitive test to demonstrate the suitability of the artificial soil for allowing the test to comply with the validity criteria unless the peat contents lowered more than specified above.
Prepare the artificial soil at least three days prior to start the test, by mixing the dry constituents listed above thoroughly in a large-scale laboratory mixer. A portion of the deionized water required is added while mixing is continued. Allowance should be made for any water that Is used for introducing the test substance into the soil. The amount of calcium carbonate required can vary, depending on properties of the individual batch of sphagnum peat and should be determined by measuring sub-samples immediately before the test (see Annex C). Store the mixed artificial soil at room temperature for at least two days to equilibrate acidity. To determine pH and the maximum water holding capacity, the dry artificial soil is pre-moistened one or two days before starting the test by adding deionised water to obtain approximately half of the required final water content of 40 % to 60% of the maximum water holding capacity.
The total water-holding capacity shall be determined in accordance with Annex B, the pH shall be determined according to ISO 10390.
5.3 Reference substance
5.3.1 General. To ensure the quality of the test system, tests should be performed regularly (once or twice a year) with a reference substance.
The NOEC and/or the EC of a reference substance shall be determined to provide assurance that the laboratory test conditions are adequate and to verify that the response of the test organisms did not change over time. The reference substance can be tested in parallel to the determination of the toxicity of each test sample at one concentration, which needs be demonstrated beforehand in a dose response study to result in an effect of about 50 %. In this case, the number of replicates should be the same as that in the controls. Alternatively, the reference substance is tested once or twice a year in a dose- response test. Depending on the design chosen, the number of concentrations and replicates and the spacing factor differ (see 2,1.3), but a response of 10 % to 90 % effect should be achieved (spacing factor of 1,8). Dimethoate as well as boric acid are suitable reference substances that have shown to affect reproduction[Z51.
The EC50 for dimethoate based on the number of juveniles should fall in the range between 3,0 mg a.s. (active substance)/kg soil (dry mass) and 7,0 mg a.s. (active substance)/kg soil (dry mass). Based on the results obtained with boric acid so far, the EC50 based on the number of juveniles should fall in the range between 100 mg/kg (dry mass) soil and 300 mg/kg (dry mass) soil.BS ISO 21285 pdf download.


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