ISO 10932-2010 pdf download.Milk and milk products – Determination of the minimal inhibitory concentration (MIC) of antibiotics applicable to bifidobacteria and non-enterococcal lactic acid bacteria (LAB).
ISO 10932 specifies a method for determining the minimal inhibitory concentration (MIC) of a series of antibiotics applicable to bifidobacteria and non-enterococcal lactic acid bacteria (LAB).
NOTE Unlike the disk diffusion method, which is semi-quantitative, the frequently used broth microdilution method gives quantitative MlCs of the test organism in a dilution series of the antibiotics. The lowest concentration of an antibiotic that prevents visible growth of a test organism is considered to be the MIC.
This International Standard recommends the broth microdilution method as the standard method.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 6887-5, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 5: Specific rules for the preparation of milk and milk products
ISO 7218, Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations
ISO/IS 11133-1, Microbiology of food and animal feeding stuffs — Guidelines on preparation and production of culture media — Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
ISO/IS 11133-2, Microbiology of food and animal feeding stuffs — Guidelines on preparation and production of culture media — Part 2: Practical guidelines on performance testing of culture media
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1 minimal inhibitory concentration MIC lowest concentration that, under defined in vitro conditions, prevents visible growth of bacteria within a defined period of time
[ISO 207761:2006(6), 24]
NOTE MIC is exp’essed in micrograms per millilitre.
4 Principle
Most individual colonies from an agar plate are picked up and suspended in sterile saline. However, Bifidobacterium spp. are suspended in pre-reduced LSM-Cys medium.
The bacterial suspension is diluted with recommended medium.
The microdilution plate is prepared with a series of twofold dilutions of antibiotic.
The diluted bacterial suspension is distributed into the wells of the plate and incubated under recommended conditions.
The lowest concentration of an antibiotic that prevents visible growth is considered to be the MIC.
5 Diluents, culture media and reagents
5.1 Basic materials
Use only reagents of recognized analytical grade. unless otherwise specified, and sterile distilled or
demineralized water or water of equivalent purity. See ISO 6887-5.
5.2 Diluents
See ISO 6887-5.ISO 10932 pdf free download.
Tags: Milk and milk products
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